1. By looking at a plant
externally can you tell whether a plant is C3 or C4 ?
Why and how?
Solution: It is not
possible to distinguish externally between a C3 and C4 plant, but
generally tropical plants are adapted for C4 cycle.
2. By looking at which
internal structure of a plant can you tell whether a plant is C3 or
C4 ?
Explain.
Solution: C4
plants live in hot moist or arid and nonsaline or saline habitats. Internally
the leaves show kranz anatomy. In kranz anatomy, the mesophyll is
undifferentiated and its cells occur in concentric layers around vascular
bundles. Vascular bundles are surrounded by large sized bundle sheath cells
which are arranged in a wreath-like manner (kranz – wreath). The mesophyll and
bundle sheath cells are connected by plasmodesmata or cytoplasmic bridges. The
chloroplasts of the mesophyll cells are smaller. They have well developed grana
and a peripheral reticulum but no starch. Mesophyll cells are specialised to
perform light reaction, evolve 02 and produce assimilatory power (ATP and
NADPH). They also possess enzyme PEPcase for initial fixation of CO2
The chloroplasts of the bundle sheath cells are agranal.
3. Even though very few cells
in a C4 plant carry out the biosynthetic – Calvin pathway, yet
they are highly productive. Can you discuss
why?
Solution: Since, through
C4 cycle, a plant can photosynthesise even in presence of very
low concentration of CO2 (upto 10 parts per million), the partial
closure of stomata due to xeric conditions would not bring much
effect.
Therefore, the plants can adapt to grow at low water content, high temperature
and bright light intensities. This cycle is specially suited to such plants
which grow in dry climates of tropics and subtropics. Besides, the
photosynthetic rate remains higher due to absence of photorespiration in these
plants. It can be visualised that both C4 cycle and
photorespiration are the result of evolution or might have been one of the
reasons of evolution for the adaptation of plants to different environments.
C4 plants are about twice to efficient as
C3 plants in converting solar energy into production of dry
matter.
4. RuBisCO is an enzyme that
acts both as a carboxylase and oxygenase. Why do you think RuBisCO carries out
more carboxylation in C4
plants?
Solution: RuBisCO is an enzyme
which acts both as carboxylase (carboxylation during photosynthesis) and
oxygenase (during photorespiration). But RuBisCO carries out more carboxylation
in C4 plants. In C4 plants, initial fixation of carbon dioxide
occurs in mesophyll cells. The primary acceptor of C02 is phosphoenol pyruvate
or PER It combines with carbon dioxide in the presence of PEP carboxylase or
PEPcase to form oxaloacetic acid or oxaloacetate. Malic acid or aspartic acid is
translocated to bundle sheath cells through plasmodesmata. Inside the bundle
sheath cells they are decarboxylated (and deaminated in case of aspartic acid)
to form pyruvate and CO2 . CO2 is again fixed inside the
bundle sheath Cells through Calvin cycle. RuBP of Calvin cycle is called
secondary or final acceptor of CO2 in C4 plants.
Pyruvate is sent back to mesophyll cells.
5. Suppose there were plants
that had a high concentration of chlorophyll b, but lacked chlorophyll a, would
it carry out photosynthesis? Then why do plants have chlorophyll b and other
accessory pigments?
Solution: Plants
that do not possess chlorophyll a will not carry out photosynthesis because it
is the primary pigment and act as the reaction centre. It performs the primary
reactions of photosynthesis or conversion of light into chemical or electrical
energy. Other photosynthetic pigments are called accessory pigments. They absorb
light energy of different wavelengths and hence broaden the spectrum of light
absorbed by photosynthetic pigments. These pigments hand over the absorbed
energy to chlorophylla.
6. Give comparison between
the following:
(a) C3
andC4 pathways
(b) Cyclic and non-cydic photophosphorylation
(c) Anatomy of leaf in C3 and C4
plants.
Solution: (a) The differences
between C3 and C4
(b) The differences between cyclic and non- cyclic
photophosphorylation are as follows :
(c) Differences between the leaf anatomy of C3 and
C4plants are as follows :
7. Look at leaves of the same
plant on the shady side and compare it with the leaves on the sunny side. Or
ompare the potted plants kept in the sunlight with those in the shade. Which of
them has leaves that are darker green?
Why?
Solution: The leaves of the shaded
side are darker green than those kept in sunlight due to two reasons:
(i) The
chloroplasts occur mostly in the mesophyll cells along their walls for receiving
optimum quantity of incident light.
(ii)The chloroplasts align themselves in
vertical position along the lateral walls of high light intensity and along
tangential wails in moderate light.
8. The given figure shows the
effect of light on the rate of photosynthesis. Based on the graph, answer the
following questions.
(a) At which point/s (A, B or C) in the curve is light
limiting factor?
(b) What could be the limiting factor/s in region A?
(c)
What do C and D represent on the curve?
Solution: (a)
At regions A and B light is the limiting factor.
(b) In the region A’, light
can be a limiting factor.
(c) C is the region where the rate of
photosynthesis is not increased when light intensity is increased. D is the
point where some other factors become limiting.
9. Why is the colour of a
leaf kept in the dark frequently becomes yellow, or pale green? Which pigment do
you think is more
stable?
Solution: Carotenoid pigments
are found in all photosynthetic cells. They are accessory pigments also found in
roots, petals etc. These pigments do not breakdown easily thus temporarily
reveal their colour due to unmasking, following breakdown of chlorophylls. Thus
the colour of leaf kept in dark is yellow or pale green.