1.’All elements that are
present in a plant need not be essential to its survival’.
Comment.
Soln. Most of the mineral
elements present in the soil enter plants through roots but all of these may not
be essential for their survival. Some are absorbed and accumulated by plant only
because they are present in excess amount. For example plants growing near
nuclear test sites take up strontium, even though it is not required by them.
Thus, an essential element is that which is necessary for supporting normal
growth and reproduction, its requirement must be specific i.e. its deficiency
cannot be met by supplying other element and it must be directly involved in the
metabolism of plant.
2.Why is purification of
water and nutrient salts so important in studies involving mineral nutrition
using hydroponics?
Soln.Impure water and
salts contain a large number of soluble minerals and impurities. When such water
and salts are used as solution culture for growing plants in hydroponics then
the impurities will interfere with the experiment and will not give correct
result about the essentiality of a mineral element. Therefore, purified water
with defined mineral nutrients are used in hydroponics.
3.Explain with
examples:macronutrients, micronutrients, beneficial nutrients, toxic elements
and essential elements.
Soln. Macronutrients :
Those elements which are generally present in plant tissues in large amounts (in
excess of 10 mmole Kg-1 of dry matter) and are involved in the
synthesis of organic molecules and development of osmotic potential are called
macronutrients or macroelement, e.g. carbon, hydrogen, oxygen, nitrogen,
sulphur, potassium, calcium and magnesium etc.
Micronutrients : Those
elements which are required by plants in’very small amounts (less than 10 mmole
Kg-1 of dry matter) are called micronutrients, e.g. iron, zinc,
manganese, boron, copper, molybdenum, chlorine and nickel. These elements are
mostly involved in the functioning of enzymes as cofactor or metal
activators.
Beneficial nutrients : Those elements which are required by
higher plants along with the macro and micronutrients are called beneficial
nutrients, e.g. cobalt, silicon, sodium and selenium.
Toxic elements : Any
mineral element if supplied to plant tissue in such concentration that it
reduces the dry weight of tissues by about 10 percent, is called toxic element.
e.g. manganese toxicity leads to the appearance of brown spots surrounded by
chlorotic veins. Excess of manganese induces deficiency of iron, magnesium and
calcium.
Essential elements : Any element required by living organisms to
ensure normal gfbwth, development, maintenance, metabolism and causes deficiency
symptoms if not supplied to the plant from external medium is called essential
element, e.g. C, H, O, N, P, K, S, Mg, Ca, Mn, Cu, Mo, Zn, B, Cl, etc. Potassium
plays an important role in opening and closing of stomata, protein synthesis
etc. Magnesium is found in chlorophyll and phosphorus in ATP. Mg2+
is an activator for both ribulose bisphosphate carboxylase-oxygenase and
phosphsenol pyruvate carboxylase.Zn2+ is an activator of alcohol
dehydrogenase and Mo of nitrogenase during nitrogen metabolism.
4.Name at least five
different deficiency symptoms in plants. Describe them and correlate them with
the concerned mineral deficiency.
Soln.Five
different deficiency symptoms in plants are:
(i)Chlorosis – It is the loss of
chlorophyll leading to yellowing of leaves. This is caused due to the deficiency
of N, K, Mg, S and Fe etc.
(ii)Necrosis – Killing or death of tissue
particularly leaf is called necrosis. This is caused due to the deficiency of
Ca, Mg, Cu and K etc.
(iii)Whiptail – Degeneration of lamina but not of
petiole and midrib , caused by deficiency of molybdenum.
(iv)Die back – It is
the killing of shoot apex i.e. stem tip and young leaves. This is caused due to
the deficiency of K and Cu.
(v)Little leaf disease – Small sized leaves,
caused by zinc deficiency.
5.If a plant shows a symptom
which could develop due to deficiency of more than one nutrient, how would you
find out experimentally, the real deficient mineral
element?
Soln.Deficiency symptoms
are first studied by means of pot and culture experiments. Rapidly growing
plants which develop characteristic symptoms are used in culture experiments.
They are called test (= indicator) plants. They are then grown in soil under
test in small pots. The results are compared to know the deficiency elements.
Similar tests are performed with selected crops.
6.Why is it that in certain
plants deficiency symptoms appear first in younger parts of the plant while in
other they do so in mature organs?
Soln.
The parts of the plants that show the deficiency symptoms depend on the
mobility of the element in the plant. For elements that are actively mobilised
within the plants and exported to young developing tissues, the deficiency
symptoms tend to appear first in the older tissues. For example, the deficiency
symptoms of nitrogen, potassium and magnesium are visible first in the senescent
leaves. In older leaves,biomolecules containing these elements are broken down,
making these elements available for mobilising to younger leaves. The deficiency
symptoms tend to appear first in the young tissues whenever the elements are
relatively immobile and are not transported out of the mature organs, for
example, elements like sulphur and calcium are a part of the structural
component of the cell and hence are not easily released.
7.How are the minerals
absorbed by the plants?
Soln. Plants absorb their
mineral salt supply from the soil through the roots from the zones of elongation
and root hair. The minerals are absorbed as ions which are accumulated by the
plants against their concentration in the soil. Plant shows two phases in
mineral absorption – initial and metabolic. In the initial phase there is a
rapid uptake of ions into outer or free space of the cells (apoplast) that
comprises of intercellular spaces and cell walls. Ions absorbed in free space
are freely exchangeable, e.g., replacement of unlabelled K+ ions with labelled
K+ ions. In the metabolic phase the ions pass into inner space comprising of
cytoplasm and vacuole. In the inner space the ions are not freely exchangeable
with those of external medium. Entry of ions into outer space is passive
absorption as no energy is required for it. Absorption of ions into inner space
requires metabolic energy. It is, therefore, an active absorption. Movement of
ions into cells is called influx while movement of ions out of the cells is
called efflux.
8.What are the conditions
necessary for fixation of atmospheric nitrogen by Rhizobiuml What is their role
inN2fixation?
Soln.The conditions
necessary for nitrogen fixation by Rhizobium are :
(i) Presence of enzyme
nitrogenase.
(ii)A protective mechanism for the enzyme nitrogenase against
O2
(iii)A non-heme iron protein-ferrodoxin as an electron
carrier.
(iv)The hydrogen donating system (viz, pyruvate, hydrogen, sucrose,
glucoseetc).
(v) A constant supply of ATP.
(vi)Presence of thiamine
pyrophosphate (TPP), coenzyme-A, inorganic phosphate and Mg++ as
co-factors.
(vii)Presence of cobalt and molybdenum,
(viii) A carbon
compound for trapping
released ammonia.
In the process of biological
nitrogen fixation by free living and symbiotic nitrogen fixers, the dinitrogen
molecule is reduced step by step to ammonia (NH3) by the addition of
pairs of hydrogen atoms. The pyruvic acid mainly serves as an electron donor but
in some cases hydrogen, sucrose, glucose, etc., have also been shown to operate.
In leguminous plants, the glucose-6-phosphate molecule probably acts as a
substrate for donating hydrogen. The overall process occurs in presence of
enzyme nitrogenase, which is active in anaerobic condition. The enzyme
nitrogenase consists of two sub-units – a non-heme iron protein (or dinitrogen
reductase) and an iron molybdenum protein (Mo-Fe protein or dinitrogenase).
The Fe-protein component reacts with ATP and reduces Mo-Fe protein which then
converts N2to ammonia. The ammonia is either
directly taken by host or is converted to nitrates with the help of nitrifying
bacteria (e.g., Nitrosomonas).
9.What are the steps involved
in formation of a root nodule?
Soln.
Nodule formation involves a sequence of multiple interactions between Rhizobium
and roots of the host plant. Main stages in the nodule formation are:
(i)
Rhizobia multiply and colonise the surrounding of roots and get attached to
epidermal and root hair cells (Figure a).
(ii)The root hair curl and the
bacteria invade the root hair.
(iii)An infection thread is produced carrying
the bacteria into the inner cortex of the root (Figure b and c).
(iv)The
bacteria get modified into rod-shaped bacteroids and cause inner cortical and
pericycle cells to divide. Division and growth of cortical and peri cycle cells
lead to nodule formation.
(v) The nodule thus formed, establishes a direct
vascular connection with the host for exchange of nutrients (Figure d).
(vi)The nodule contains all the necessary biochemical components, such as the
enzyme nitrogenase and leghaemoglobin. The enzyme nitrogenase catalyses the
conversion of atmospheric nitrogen to ammonia, the first stable product of
nitrogen fixation.
10.Which of the following
statements are true?
If false, correct
them.
(a)
Boron deficiency leads to stout axis.
(b) Every mineral element that is present in a cell is needed by the
cell.
(c)
Nitrogen as a nutrient element, is highly immobile in
plants.
(d)
It is very easy to establish the essentiality of micronutrients because they are
required only in trace quantities.
Soln. (a)
True.
(b) False. Every mineral element that is present in a cell is
not needed by the cell.
(c) False. Nitrogen as a nutrient element is highly
mobile in plants.
(d) False. It is very difficult to establish the
essentiality of micronutrients because they are required only in trace
quantities.